FastQCFastQC Report
Fri 10 Feb 2017
ERR1147861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219507
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144100.4475840555712412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87440.2715943776485033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75280.23382461973215155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47850.068.317011
GTATCAA99350.062.930311
TCAACGC136650.045.352784
ATCAACG136750.045.3196143
CAACGCA139400.044.4580845
AACGCAG140550.044.0943226
TATCAAC143950.043.5161252
ACGCAGA164550.037.554637
GTACATG174350.036.2011531
CGCAGAG172550.035.916888
TACATGG178200.035.0188032
ACATGGG179900.033.854363
GCAGAGT185100.033.449549
CATGGGT55550.031.4781424
CATGGGG101300.030.6483364
AGAGTAC175550.028.45930310-11
GAGTACT82000.026.2929912-13
ATGGGGG57250.026.2839645
CAGAGTA181350.026.22084810-11
AGTACTT86050.025.88491612-13
Produced by FastQC (version 0.11.5)