FastQCFastQC Report
Fri 10 Feb 2017
ERR1147860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962952
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143870.732926734836104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93310.47535548500421815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79250.4037286698808733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20960.1067779548353704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48600.066.411131
GTATCAA93450.057.223561
ATCAACG127050.041.709843
TCAACGC127900.041.5256464
TATCAAC130350.040.9816362
AACGCAG129550.040.9049386
CAACGCA131200.040.571845
ACGCAGA150250.035.190287
CGCAGAG152550.034.7376948
GTACATG142500.033.1805421
CATGGGT45000.032.9095764
TACATGG143750.032.6507452
GCAGAGT163300.032.523769
ACATGGG143900.032.320753
ATGGGTG23350.030.8200935
CATGGGG73150.029.3513684
AGAGTAC159500.028.13416510-11
AGTACTT78250.027.66639112-13
GAGTACT77250.027.25462712-13
ATGGGGG38750.026.7062555
Produced by FastQC (version 0.11.5)