FastQCFastQC Report
Fri 10 Feb 2017
ERR1147859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505585
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92660.36981383589062033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62160.24808577637557694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46640.18614415396005324No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC34920.13936865043492838No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT34530.13781212770670323No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT32390.12927120812105755No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA31020.12380342315267692No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC29070.11602080951155118No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG28680.11446428678332606No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA28270.11282794237673038No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT26190.10452648782619627No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA25930.10348880600737952No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC25720.10265067838448905No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA25400.10137353153056074No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC25330.10109415565626391No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG25290.10093451229952285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.060.553061
GTATCAA79100.057.0002671
TCAACGC106900.041.898044
CAACGCA109400.040.9405945
ATCAACG109800.040.8997883
AACGCAG111400.040.5259326
TATCAAC126850.035.6503872
ACGCAGA128950.035.0103877
CGCAGAG132200.034.10478
GCAGAGT143400.031.482499
GTACATG132750.030.1054761
TACATGG135700.029.335092
ACATGGG134550.028.9999053
CATGGGT29900.027.2537214
GAGTACT53800.027.14223912-13
AGAGTAC135250.026.38702610-11
CATGGGG71650.025.485824
CAGAGTA139300.025.36365310-11
AGTACTT56500.024.68714512-13
GTACTTT60600.024.24380514-15
Produced by FastQC (version 0.11.5)