FastQCFastQC Report
Fri 10 Feb 2017
ERR1147858.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147858.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485104
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39930.26887005893189975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27160.1828828149409065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18160.1222809985024618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31800.056.553871
GGTATCA17500.052.7443771
TCAACGC40750.043.207974
CAACGCA41200.042.7360345
ATCAACG41950.041.8301853
AACGCAG43600.040.6564526
TATCAAC48900.036.619792
ACGCAGA51400.034.4867977
CGCAGAG53650.032.707858
GCAGAGT61550.028.7030549
GTACATG56900.026.6876561
TACATGG58350.025.8970972
ACATGGG57300.025.8490433
AGAGTAC55550.025.21776610-11
CAGAGTA57950.023.40351710-11
CATGGGG33450.022.0508464
AGTACTT28500.021.70642312-13
GAGTACT26800.021.30768212-13
GTAAGTA5750.020.6928542
GTACTTT27750.020.68540614-15
Produced by FastQC (version 0.11.5)