FastQCFastQC Report
Fri 10 Feb 2017
ERR1147856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402993
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67280.27998416974165136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41880.17428265500565335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35240.14665044800380192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.062.856711
GTATCAA52900.056.959391
TCAACGC69600.042.9846954
CAACGCA70600.042.3758475
ATCAACG73150.040.8173183
AACGCAG73800.040.6190076
TATCAAC83200.036.3233452
ACGCAGA86900.034.4957737
GTACATG95950.033.8237721
CGCAGAG91850.032.5719648
TACATGG101000.031.865552
ACATGGG99700.031.6777613
GCAGAGT100400.029.8573979
CATGGGG61450.026.1334634
AGAGTAC92150.026.0760110-11
CATGGGT27850.024.9870954
CAGAGTA96650.024.80037910-11
GAGTACT43300.023.35157412-13
ATGGGGG29500.022.7830335
TATACCG1903.3170916E-521.9128845
Produced by FastQC (version 0.11.5)