FastQCFastQC Report
Fri 10 Feb 2017
ERR1147855.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147855.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345831
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58700.2502311547592303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.14813513846479137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29540.12592552489927877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48100.063.3789331
GGTATCA23400.059.0326351
TCAACGC64250.046.5582054
ATCAACG66250.045.242443
CAACGCA66000.045.2335975
AACGCAG68050.044.0457236
TATCAAC76550.039.4758032
ACGCAGA82850.036.249357
CGCAGAG86850.034.648318
GTACATG95850.033.2339321
TACATGG98200.032.1658822
ACATGGG98300.031.7619483
GCAGAGT96300.031.1864859
CATGGGG58000.026.8642464
AGAGTAC88550.026.32684910-11
CATGGGT25650.025.0401974
CAGAGTA92700.023.60855710-11
GAGTACT36500.023.46232212-13
GTATATA6100.023.4261781
GTCTTAG8200.022.5096071
Produced by FastQC (version 0.11.5)