FastQCFastQC Report
Fri 10 Feb 2017
ERR1147854.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147854.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3971626
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120170.30257128944165435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66690.16791611294719092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58700.14779840800719907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.060.581541
GTATCAA89950.060.175441
TCAACGC115950.046.4233634
CAACGCA118400.045.462745
ATCAACG119550.044.975673
AACGCAG122800.043.882226
TATCAAC139000.038.8627662
ACGCAGA145450.037.0078287
CGCAGAG151750.035.3930368
GTACATG158500.033.361091
GCAGAGT167250.032.1840869
TACATGG163850.031.9883692
ACATGGG163550.031.2393043
CATGGGG94950.026.9985894
AGAGTAC156850.026.86668410-11
CAGAGTA164200.024.34192310-11
GAGTACT72850.023.92196312-13
AGTACTT78150.022.79431712-13
CATGGGT40650.022.2403584
ATGGGGG46450.021.8962215
Produced by FastQC (version 0.11.5)