FastQCFastQC Report
Fri 10 Feb 2017
ERR1147853.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147853.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983961
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108540.36374470041666096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64460.2160215900945086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56540.18947968823989322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.065.011031
GTATCAA76550.058.5041961
TCAACGC102900.043.1191144
CAACGCA105800.042.0496445
ATCAACG106500.041.7174153
AACGCAG106800.041.5445376
TATCAAC118750.037.827932
ACGCAGA128050.034.650197
CGCAGAG133150.033.4123348
GTACATG129700.032.4633871
TACATGG134050.031.1136132
GCAGAGT146100.030.6135739
ACATGGG134950.030.4985773
CATGGGG80950.028.287224
AGAGTAC134900.027.02681510-11
CAGAGTA141750.024.44101510-11
GAGTACT65100.023.34298112-13
AGTACTT68800.023.25467312-13
CATGGGT33950.022.9497454
GTCTTAG10200.022.186961
Produced by FastQC (version 0.11.5)