FastQCFastQC Report
Fri 10 Feb 2017
ERR1147852.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147852.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875341
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64120.3419111510919881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39700.21169483309968692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29110.15522510306125661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23650.068.727471
GTATCAA47300.062.3078731
TCAACGC64200.045.3962174
CAACGCA65950.044.191625
ATCAACG66050.044.124713
AACGCAG68100.042.709096
TATCAAC72250.040.6751172
ACGCAGA79100.036.844977
CGCAGAG82600.035.211748
GCAGAGT90100.032.676769
GTACATG79450.030.2002141
TACATGG83200.028.6721822
ACATGGG83800.027.7518143
GAGTACT38600.027.04259912-13
AGAGTAC84850.026.60226210-11
CAGAGTA88300.025.69759410-11
GTACTTT43900.023.91326114-15
CATGGGG48500.023.055514
CATGGGT22450.023.0495014
AGTACTT41950.023.03987112-13
Produced by FastQC (version 0.11.5)