FastQCFastQC Report
Fri 10 Feb 2017
ERR1147851.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147851.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588200
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87560.3383046132447261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59520.2299667722741674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43060.16637045050614327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.070.593041
GTATCAA65600.059.290491
TCAACGC89100.043.993384
ATCAACG89200.043.9440573
CAACGCA91600.042.9874955
AACGCAG96050.041.057816
TATCAAC101600.038.5964662
ACGCAGA112050.035.1950237
CGCAGAG118350.033.2712678
GCAGAGT129600.030.337249
GTACATG112050.029.0771051
TACATGG117450.027.5614262
ACATGGG116400.027.1855583
AGAGTAC118250.026.40813810-11
CATGGGG69450.025.8651084
CAGAGTA123550.025.05864510-11
GAGTACT55700.025.04189712-13
AGTACTT60250.022.7558712-13
GTACTTT61750.022.54023714-15
GTAAGTA14000.022.5270182
Produced by FastQC (version 0.11.5)