FastQCFastQC Report
Fri 10 Feb 2017
ERR1147850.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147850.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043952
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117330.3854528586521732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64150.21074576734455733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60410.198459108422209No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78650.061.642291
GGTATCA39150.054.9197431
TCAACGC103350.046.2684174
CAACGCA104850.045.5497745
AACGCAG107550.044.6274726
ATCAACG107300.044.5097273
TATCAAC116950.041.001752
ACGCAGA127050.037.7311027
CGCAGAG133250.035.9308788
GCAGAGT145750.032.9309359
GTACATG130150.031.4845391
ACATGGG131800.030.5500813
TACATGG134800.030.1879582
CATGGGG78750.028.7748814
AGAGTAC133200.028.42060310-11
AGTACTT69850.025.33142912-13
GAGTACT65050.025.28057512-13
CAGAGTA139950.024.92494610-11
ATGGGGG40350.023.5838975
GTACTTT70300.023.51952714-15
Produced by FastQC (version 0.11.5)