FastQCFastQC Report
Fri 10 Feb 2017
ERR1147849.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147849.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3521827
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119790.3401359578423358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72910.20702322970435516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57590.1635230804920287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43600.062.6940421
GTATCAA85900.058.373941
TCAACGC112150.044.230084
CAACGCA114650.043.2137455
ATCAACG117000.042.346383
AACGCAG119350.041.511996
TATCAAC129800.038.8268662
ACGCAGA140650.035.183147
CGCAGAG145950.033.9055028
GCAGAGT159700.031.0235219
GTACATG147550.030.7953681
TACATGG152150.029.7993432
ACATGGG150200.029.6202563
AGAGTAC147900.026.36061310-11
GAGTACT68950.025.61964812-13
CATGGGG94800.025.535074
CAGAGTA155200.024.69916210-11
GTACTTT74350.023.99889414-15
AGTACTT73950.023.8069912-13
ATGGGGG52950.020.8928645
Produced by FastQC (version 0.11.5)