FastQCFastQC Report
Fri 10 Feb 2017
ERR1147848.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147848.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217916
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116260.3612897291290388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72360.22486603130721872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58960.18322417365773377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49050.059.4853821
GTATCAA92400.057.9994281
TCAACGC123200.042.819954
ATCAACG126300.041.8160363
CAACGCA128100.041.2748875
AACGCAG133150.039.7541166
TATCAAC139550.037.940952
ACGCAGA156350.033.8932387
CGCAGAG163400.032.467298
GTACATG170150.031.321621
TACATGG175550.030.4315092
GCAGAGT175750.030.2873279
ACATGGG172300.030.2034133
CATGGGT48000.028.4983624
CATGGGG97700.026.2979854
AGAGTAC164450.025.67779410-11
GAGTACT69600.025.63570612-13
CAGAGTA171950.024.0389710-11
GTACTTT74400.023.9418214-15
AGTACTT70900.023.57183312-13
Produced by FastQC (version 0.11.5)