FastQCFastQC Report
Fri 10 Feb 2017
ERR1147847.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147847.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3703576
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107580.29047601561301833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63860.17242794531555447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53250.14377995753293574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41800.062.6999361
GTATCAA81800.060.2931181
TCAACGC108250.045.056214
CAACGCA111250.043.894685
ATCAACG112150.043.4899753
AACGCAG115950.042.115426
TATCAAC127950.038.4151042
ACGCAGA138950.035.3154037
CGCAGAG144450.033.9295778
GTACATG150100.031.2309171
GCAGAGT158300.030.8482829
TACATGG156800.029.6392522
ACATGGG154250.029.4606823
AGAGTAC145600.026.39002810-11
GAGTACT63650.025.60476912-13
CATGGGG90300.024.6350024
CAGAGTA152800.024.46530510-11
AGTACTT71200.023.22381412-13
GTACTTT72400.022.46919414-15
CATGGGT40150.022.073414
Produced by FastQC (version 0.11.5)