FastQCFastQC Report
Fri 10 Feb 2017
ERR1147846.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147846.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791838
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73640.2637688863035749No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42450.15205036968477398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36400.13038005786868723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27200.065.4506451
GTATCAA56900.061.2146261
TCAACGC76800.044.7581754
ATCAACG79700.042.9803433
CAACGCA80050.042.866725
AACGCAG82000.041.77486
TATCAAC94950.036.335582
ACGCAGA95700.035.7323577
GTACATG111400.034.259771
CGCAGAG102100.033.492528
TACATGG114900.032.8222472
ACATGGG114200.032.2351953
GCAGAGT114700.029.8651569
CATGGGG69900.029.0974334
AGAGTAC103350.026.64257810-11
CAGAGTA109050.023.80478910-11
ATGGGGG37550.023.4399935
AGTACTT51000.023.03046212-13
TATACCG3103.4015102E-1023.0210595
GAGTACT49350.023.01717412-13
Produced by FastQC (version 0.11.5)