FastQCFastQC Report
Fri 10 Feb 2017
ERR1147845.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147845.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305641
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60690.26322397979564033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34620.1501534714207459No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28820.12499777719081158No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT25570.1109019140447277No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC23500.10192393351783735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21000.062.6804581
GTATCAA43400.061.6191641
TCAACGC58700.044.7896734
CAACGCA59050.044.4234625
ATCAACG61200.043.0581553
AACGCAG61200.042.960036
TATCAAC74200.035.926022
ACGCAGA74400.035.4180377
GTACATG86400.034.743691
TACATGG90550.033.053282
CGCAGAG80000.033.013138
ACATGGG92100.031.71213
GCAGAGT89100.029.9752189
CATGGGT30000.027.3622344
AGAGTAC79050.027.12670710-11
CATGGGG53050.025.0042134
CAGAGTA83550.023.7434110-11
AGTACTT37350.022.29621712-13
GAGTACT35450.021.98106812-13
AGTACAT63150.021.1935112-13
Produced by FastQC (version 0.11.5)