FastQCFastQC Report
Fri 10 Feb 2017
ERR1147844.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147844.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616913
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56470.2157886028308927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33870.12942730614277204No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT30820.11777235238618938No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC28360.10837196345465057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.10271644491047277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50550.059.2521171
GGTATCA24950.057.9952931
TCAACGC68400.043.4758264
CAACGCA69800.042.603825
ATCAACG71450.041.786453
AACGCAG74200.040.0774466
GTACATG115350.037.477971
TACATGG117350.036.7040442
ACATGGG118600.035.8554573
TATCAAC84350.035.8291932
CATGGGT45450.034.153924
ACGCAGA89550.033.2740827
CGCAGAG94500.031.5311558
CATGGGG61000.029.0549664
GCAGAGT105450.028.369749
ATGGGTG25450.028.043195
AGAGTAC95100.025.64113610-11
ATGGGGG31450.023.0713585
CAGAGTA99650.022.47096310-11
GAGTACT37850.021.99865512-13
Produced by FastQC (version 0.11.5)