FastQCFastQC Report
Fri 10 Feb 2017
ERR1147843.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147843.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430209
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82580.33980616481956905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52930.2178001974315789No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42280.17397680611009175No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT24450.1006086307803156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32500.065.042081
GTATCAA62650.058.072381
TCAACGC84350.042.870484
CAACGCA87300.041.35375
ATCAACG88700.040.8350943
AACGCAG88950.040.5197336
TATCAAC99950.036.3675842
ACGCAGA108250.033.1855437
CGCAGAG112400.032.013198
GTACATG113950.030.8309691
TACATGG119300.029.2222212
GCAGAGT124000.029.1143349
ACATGGG119150.028.9017683
CATGGGG65950.027.505844
AGAGTAC114050.025.1618110-11
GAGTACT48500.024.83265512-13
CAGAGTA118100.024.22339810-11
ATGGGGG32250.023.2370365
AGTACTT51600.022.87971912-13
GTACTTT55100.022.02005814-15
Produced by FastQC (version 0.11.5)