FastQCFastQC Report
Fri 10 Feb 2017
ERR1147842.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147842.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035664
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100160.32994428895951594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55540.1829583247684856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50910.16770630741742168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66750.066.001151
GGTATCA30950.059.823361
TCAACGC91100.047.591494
CAACGCA93500.046.3062865
ATCAACG94500.045.8792083
AACGCAG96800.044.727666
TATCAAC107150.040.802512
ACGCAGA113250.038.3358277
CGCAGAG118150.036.7962728
GCAGAGT130900.033.212229
GTACATG120750.032.6886861
ACATGGG124350.030.9441573
TACATGG127200.030.8169772
AGAGTAC119650.028.72997910-11
CATGGGG78850.026.3989414
GAGTACT56050.025.41265712-13
CAGAGTA124100.025.06398410-11
AGTACTT59800.024.9130412-13
TAAACCG2001.8347127E-623.7892175
GTACTTT62350.022.89259114-15
Produced by FastQC (version 0.11.5)