FastQCFastQC Report
Fri 10 Feb 2017
ERR1147841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3243390
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109230.3367772608289475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61820.19060304187902163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52870.163008457200645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78350.063.8414731
GGTATCA36400.062.0012471
TCAACGC103400.047.802884
CAACGCA106200.046.4865385
ATCAACG107200.046.219353
AACGCAG109400.045.126786
TATCAAC118900.041.933682
ACGCAGA129900.037.7762157
CGCAGAG136450.036.006438
GCAGAGT150900.032.6373379
GTACATG138650.030.6648561
TACATGG142300.029.3934942
ACATGGG141600.029.1100923
AGAGTAC137600.027.66528110-11
CATGGGG88200.026.5705914
CAGAGTA144250.024.82310-11
GAGTACT62300.024.29815712-13
AGTACTT66650.024.0955712-13
GTACTTT68900.022.10010514-15
GTAAGTA16350.021.8340512
Produced by FastQC (version 0.11.5)