FastQCFastQC Report
Fri 10 Feb 2017
ERR1147840.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147840.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551526
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66650.26121622903313546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37900.1485385608455489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33500.13129397858379652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51050.061.2311441
GGTATCA26200.059.9945031
TCAACGC70200.044.4789124
CAACGCA70250.044.4472585
ATCAACG73300.042.678953
AACGCAG74050.042.407326
TATCAAC84500.037.4590872
ACGCAGA87200.035.875777
CGCAGAG92400.033.7280548
GTACATG98850.032.826811
TACATGG104100.031.1492772
GCAGAGT102750.030.4463949
ACATGGG105400.030.1323243
AGAGTAC92050.027.20133810-11
CATGGGG64450.027.1304134
CAGAGTA96700.024.44796210-11
GAGTACT42650.022.9392312-13
GTGTACG3900.022.9000021
ATGGGGG36300.022.1186775
GTAAGTA13200.021.6356282
Produced by FastQC (version 0.11.5)