FastQCFastQC Report
Fri 10 Feb 2017
ERR1147839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3403825
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103950.30539172842317097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63060.18526216829596118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49540.14554214743707447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38400.061.105311
GTATCAA76950.060.8314061
TCAACGC102500.044.916014
CAACGCA104900.043.831685
ATCAACG105150.043.7274633
AACGCAG108700.042.3541036
TATCAAC122050.037.97342
ACGCAGA127800.035.9311037
CGCAGAG133550.034.4286278
GTACATG136600.032.7854461
TACATGG141050.031.719372
ACATGGG141300.031.1090223
GCAGAGT150100.030.9495589
AGAGTAC135650.027.31821810-11
CATGGGG85350.025.855594
GTATACG2151.2226519E-724.9297541
CAGAGTA142800.024.3883710-11
GAGTACT63600.024.22293312-13
AGTACTT68100.022.84066212-13
GTACTTT70200.022.07266214-15
Produced by FastQC (version 0.11.5)