FastQCFastQC Report
Fri 10 Feb 2017
ERR1147838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437853
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67320.2761446239785582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42590.17470290456397494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33660.1380723119892791No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49650.062.9725381
GGTATCA24800.061.95541
TCAACGC66850.046.083164
CAACGCA68200.045.2581635
ATCAACG69500.044.4971773
AACGCAG71700.043.048916
TATCAAC81750.038.492042
ACGCAGA87250.035.376587
CGCAGAG91500.033.8633968
GTACATG91950.031.2180981
GCAGAGT102550.030.1565489
TACATGG96650.029.5421712
ACATGGG94800.029.4224223
CATGGGT30900.028.1001284
AGAGTAC92100.027.25001310-11
CATGGGG52750.023.7888744
CAGAGTA97800.023.71590410-11
GAGTACT43800.023.28648612-13
GTATAGG6000.022.8290421
AGTACTT47800.022.39538412-13
Produced by FastQC (version 0.11.5)