FastQCFastQC Report
Fri 10 Feb 2017
ERR1147836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198424
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69950.3181824798128114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45270.20592024104540343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.15738547250211968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.067.149171
GTATCAA55550.060.5557331
TCAACGC74700.044.667174
CAACGCA76550.043.5099955
ATCAACG77700.042.712933
AACGCAG78950.042.1873366
TATCAAC84000.039.7999232
ACGCAGA92100.036.228427
CGCAGAG95300.034.8247078
GCAGAGT104900.031.63779
GTACATG92100.028.572851
AGAGTAC97350.027.12646710-11
TACATGG96350.027.1042882
ACATGGG97750.026.346163
CAGAGTA101050.025.83892310-11
GAGTACT46500.025.00575612-13
GTACTTT50300.023.41226214-15
AGTACTT49650.023.0000212-13
CATGGGT27550.022.452154
CATGGGG54950.020.7816374
Produced by FastQC (version 0.11.5)