FastQCFastQC Report
Fri 10 Feb 2017
ERR1147834.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147834.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866411
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59310.31777566677436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.18891873226207948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30930.16571912617317408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.064.566611
GTATCAA42450.062.851491
TCAACGC57100.046.4549334
CAACGCA58150.045.616115
ATCAACG58800.045.111853
AACGCAG60000.044.4078646
TATCAAC67200.040.1009942
ACGCAGA71700.037.078447
CGCAGAG74800.035.5417678
GTACATG84150.032.9089551
TACATGG85900.032.2714842
GCAGAGT84050.031.8425569
ATGGGGG30700.031.190385
ACATGGG87750.030.9065723
CATGGGG53500.030.3488314
AGAGTAC76300.027.08715610-11
GAGTACT30150.025.84146512-13
TATACAG6700.025.7428325
TGGGGGG26100.025.2938886
TCTATAC4050.024.9647393
Produced by FastQC (version 0.11.5)