FastQCFastQC Report
Fri 10 Feb 2017
ERR1147833.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147833.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903096
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48240.25348169509052615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.14739140852589674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.12931559942325557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.066.597031
GTATCAA41050.061.7957841
TCAACGC52800.047.203524
ATCAACG54750.045.632153
CAACGCA54850.045.5477565
AACGCAG57750.043.363516
TATCAAC63150.040.6072
ACGCAGA68550.036.4448477
CGCAGAG73550.033.9672938
GTACATG77950.031.9317381
GCAGAGT78450.031.845699
TACATGG79800.031.0906752
ACATGGG82450.029.1471733
AGAGTAC73100.026.36464910-11
CATGGGG49200.025.8727744
CAGAGTA77050.024.7428510-11
AGTACAC16350.024.739843
GTAAGTA9050.023.6674212
GTACACT16500.023.4327744
GAGTACT31250.023.41258612-13
Produced by FastQC (version 0.11.5)