FastQCFastQC Report
Fri 10 Feb 2017
ERR1147832.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147832.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836535
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67740.36884676850699827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41090.22373654735684317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33880.18447783461790818No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26600.058.892471
GTATCAA50500.057.205271
TCAACGC68850.042.157464
CAACGCA70550.041.1416175
ATCAACG71100.040.9070173
AACGCAG71850.040.5627986
TATCAAC76700.037.9347842
ACGCAGA84350.034.551717
CGCAGAG87850.033.1751488
GCAGAGT95350.030.8151869
GTACATG86100.030.5085181
TACATGG88850.029.5328452
ACATGGG89050.028.7205723
AGAGTAC88600.026.95320310-11
CATGGGG55000.026.9274484
GTATCGA1150.001007183525.8601329
GAGTACT39850.024.7017812-13
CAGAGTA91750.024.47199810-11
AGTACTT41800.024.11859312-13
ATGGGGG32900.023.8636365
Produced by FastQC (version 0.11.5)