FastQCFastQC Report
Fri 10 Feb 2017
ERR1147831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860648
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61410.33004630644807614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36980.1987479630752297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29670.159460575025475No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18860.10136253606270504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.066.768861
GTATCAA45950.056.2394751
TCAACGC57700.044.427114
CAACGCA59400.043.1556245
AACGCAG60900.042.1903426
ATCAACG61200.041.7891543
TATCAAC69950.037.081912
ACGCAGA72400.035.3245357
GTACATG79250.034.03581
CGCAGAG75700.033.7846268
TACATGG81700.032.622652
CATGGGT24300.032.553074
ACATGGG83150.031.6875063
GCAGAGT82900.031.1373589
CATGGGG45100.028.0898654
GAGTACT36200.027.27382912-13
TATACTG5850.026.434075
AGAGTAC76550.026.3391110-11
CAGAGTA79850.026.33061810-11
AGTACTT37850.025.29918712-13
Produced by FastQC (version 0.11.5)