FastQCFastQC Report
Fri 10 Feb 2017
ERR1147830.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147830.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979164
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62060.31356673827939474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37730.19063604633067296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28430.14364650933424414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49700.060.6387061
GGTATCA24900.056.211391
TCAACGC64450.045.8712274
CAACGCA66000.044.703825
ATCAACG67650.044.1410643
AACGCAG68500.043.245976
TATCAAC76150.039.380142
ACGCAGA81000.036.572217
CGCAGAG86150.034.3859448
GCAGAGT91950.032.346349
GTACATG88650.031.308551
TACATGG90450.030.1940042
ACATGGG91850.029.0138743
AGAGTAC84650.027.01943610-11
CATGGGG52850.026.0000294
CAGAGTA85850.025.56777610-11
GAGTACT34650.024.46349312-13
TAGCGTG1250.001636355523.7939665
ATGGGGG27100.023.7061675
CATGGGT21350.023.682524
Produced by FastQC (version 0.11.5)