FastQCFastQC Report
Fri 10 Feb 2017
ERR1147829.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147829.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609756
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148980.5708579652657184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88260.3381925360071976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70260.269220570811984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99150.054.152811
GGTATCA52950.052.8371121
AACGCAG129800.041.059716
TCAACGC130400.040.825174
ATCAACG131700.040.467353
CAACGCA133000.039.9823575
TATCAAC140700.038.2600362
ACGCAGA153950.034.657357
CGCAGAG155950.034.1734248
GCAGAGT169950.031.533319
GTACATG138550.028.3559741
TACATGG142600.027.4471472
ACATGGG140850.027.280873
AGAGTAC160800.026.6880110-11
CATGGGG80450.025.5079524
GAGTACT83400.025.3535912-13
AGTACTT86100.025.042112-13
CAGAGTA167150.024.41089210-11
ATGGGGG42250.023.088715
GTACTTT95800.022.13414-15
Produced by FastQC (version 0.11.5)