FastQCFastQC Report
Fri 10 Feb 2017
ERR1147828.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147828.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221147
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169910.5274829121427864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92500.2871647894368062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78800.2446333557580576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.056.5677871
GTATCAA112750.055.7615661
TCAACGC149650.041.736474
AACGCAG149850.041.601376
ATCAACG153750.040.6234973
CAACGCA153900.040.58395
TATCAAC165550.038.0890432
ACGCAGA175450.035.5991027
CGCAGAG180350.034.5946168
GTACATG176150.033.1231041
TACATGG181500.032.185352
CATGGGG100750.032.177674
ACATGGG176900.032.146463
GCAGAGT196950.031.7693989
ATGGGGG53350.028.6550945
AGAGTAC186750.027.4370310-11
GAGTACT93100.025.20227612-13
CAGAGTA193250.024.72912810-11
AGTACTT99700.023.65323312-13
CATGGGT37200.022.7064134
Produced by FastQC (version 0.11.5)