FastQCFastQC Report
Fri 10 Feb 2017
ERR1147827.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147827.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402508
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94820.39467090224049206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49540.2062011864268506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45330.18867783166590912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75400.051.481371
GGTATCA41950.043.569271
TCAACGC95100.040.2784044
CAACGCA96800.039.5095945
ATCAACG97000.039.428133
AACGCAG97700.039.3282746
TATCAAC108700.035.5147862
ACGCAGA112750.034.1307337
CGCAGAG117000.032.941788
GTACATG118300.032.40971
TACATGG121400.031.4075012
ACATGGG119050.030.9263133
GCAGAGT129400.029.968939
TATACCG2751.8189894E-1228.1175585
CATGGGG64400.026.1377074
AGAGTAC120100.025.92576410-11
GAGTACT60250.025.42014712-13
CATGGGT25400.025.2904764
TATAACG1951.4695324E-624.4033472
AGTACTT64400.023.55115512-13
Produced by FastQC (version 0.11.5)