FastQCFastQC Report
Fri 10 Feb 2017
ERR1147826.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147826.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2902664
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163320.5626555467666944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90040.3101978044995907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80180.2762290089379963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109200.059.3700031
GGTATCA53550.052.918721
TCAACGC148550.043.4069484
AACGCAG148650.043.3777476
ATCAACG150500.042.9631043
CAACGCA151050.042.7672845
TATCAAC159500.040.762622
ACGCAGA172050.037.4780657
CGCAGAG177000.036.3602338
GCAGAGT192900.033.4865389
GTACATG160800.031.0625971
TACATGG166700.029.9384252
ACATGGG165050.029.5529563
AGAGTAC183600.028.67003810-11
AGTACTT97600.026.14358712-13
CATGGGT32100.025.7580134
GAGTACT92600.025.43559512-13
CATGGGG88050.025.3340424
CAGAGTA188600.024.7247610-11
GTACTTT104550.022.49987614-15
Produced by FastQC (version 0.11.5)