FastQCFastQC Report
Fri 10 Feb 2017
ERR1147825.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147825.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267856
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95090.41929469948709264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54230.23912452995251904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44320.19542687013637552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76700.052.850371
GGTATCA42750.050.404431
TCAACGC99050.040.41754
AACGCAG100500.039.8935556
ATCAACG100600.039.7947653
CAACGCA100900.039.73545
TATCAAC111100.036.408582
ACGCAGA115950.034.8343667
CGCAGAG118650.033.886048
GCAGAGT130850.030.7720769
GTACATG112150.029.297871
TACATGG114700.028.523852
ACATGGG112750.028.3840643
CATGGGG55750.026.9950734
AGAGTAC122650.025.67619710-11
GAGTACT59650.025.57462112-13
CAGAGTA126500.024.4245910-11
AGTACTT62150.023.49322512-13
TATCACG1552.1106994E-423.0265252
GTACTTT67600.022.56695414-15
Produced by FastQC (version 0.11.5)