FastQCFastQC Report
Fri 10 Feb 2017
ERR1147824.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147824.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771807
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165630.5975524269907682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92730.33454710230546353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82600.29800054621407623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52050.059.017771
GTATCAA103000.058.954471
TCAACGC137500.043.9525684
AACGCAG137950.043.8954286
ATCAACG140400.043.0447123
CAACGCA140800.042.9646765
TATCAAC152250.040.0460622
ACGCAGA160200.037.6496437
CGCAGAG164950.036.598228
GCAGAGT179550.033.6553849
GTACATG153000.031.8673731
ACATGGG156000.030.7710383
TACATGG158500.030.5859222
AGAGTAC170700.028.79755210-11
CATGGGG88850.027.7833044
ATGGGGG45050.027.4638715
GAGTACT89100.026.13366312-13
CAGAGTA176000.025.95343210-11
AGTACTT93800.025.58508712-13
CATGGGT33550.025.5307274
Produced by FastQC (version 0.11.5)