FastQCFastQC Report
Fri 10 Feb 2017
ERR1147822.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147822.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273927
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103680.4559513124211991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58340.25656056680799344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.20277695809935853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.061.5540121
GTATCAA67750.060.5433581
TCAACGC91250.044.7202154
ATCAACG93200.043.7845423
CAACGCA93700.043.55095
AACGCAG94600.043.1365666
TATCAAC105650.039.075322
ACGCAGA111950.036.2387247
CGCAGAG115500.035.17338
GCAGAGT123550.032.8334169
GTACATG112700.031.6416761
TACATGG115850.030.397532
ACATGGG116900.029.564753
CATGGGT28500.027.3425524
AGAGTAC118750.027.122510-11
CATGGGG65300.026.6000394
ATGGGGG35300.025.614245
AGTACTT61950.025.20303512-13
GAGTACT58700.024.82520712-13
CAGAGTA123300.024.55384810-11
Produced by FastQC (version 0.11.5)