FastQCFastQC Report
Fri 10 Feb 2017
ERR1147821.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147821.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088034
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202040.6542674076775061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111810.36207502896665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93600.3031054709889852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31320.1014237537540066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133900.057.120581
GGTATCA69300.051.705091
AACGCAG172850.043.8066
ATCAACG178500.042.5527153
TCAACGC178900.042.391074
CAACGCA181050.041.854825
TATCAAC190550.040.2051352
ACGCAGA202450.037.430557
CGCAGAG206500.036.666458
GCAGAGT223150.033.9839489
GTACATG186050.030.1044031
TACATGG190600.029.0849022
ACATGGG191350.028.4424613
AGAGTAC212200.028.3093210-11
GAGTACT106150.027.20337312-13
CATGGGT45250.025.763934
AGTACTT113000.025.73853912-13
CATGGGG99900.025.7212964
CAGAGTA219100.025.54469710-11
GTACTTT123300.023.4196114-15
Produced by FastQC (version 0.11.5)