FastQCFastQC Report
Fri 10 Feb 2017
ERR1147820.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147820.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3358438
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172580.5138698406818883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90330.26896432210450216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75490.22477711364628436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119600.056.6489871
GGTATCA62950.050.7878231
AACGCAG157400.042.4084326
TCAACGC158250.042.1806454
ATCAACG162000.041.2776873
CAACGCA162150.041.1661265
TATCAAC171650.039.373012
ACGCAGA184650.036.2127657
CGCAGAG189000.035.346248
GCAGAGT204750.032.6854069
GTACATG172800.029.4580121
TACATGG175850.028.689142
ACATGGG173550.028.3151933
AGAGTAC194400.027.15757210-11
GAGTACT98150.024.87950112-13
AGTACTT104900.024.78133812-13
CATGGGG91700.024.7183654
CATGGGT43600.024.561234
CAGAGTA201250.024.51880610-11
ATGGGGG43250.021.5962125
Produced by FastQC (version 0.11.5)