FastQCFastQC Report
Fri 10 Feb 2017
ERR1147819.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147819.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2674753
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118530.4431437220558309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60180.22499273764717714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55780.20854262057094616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94300.050.7488671
GGTATCA52650.044.656091
TCAACGC119650.039.4171374
AACGCAG120700.039.2713366
CAACGCA122650.038.5984735
ATCAACG123200.038.088243
TATCAAC133000.035.641472
ACGCAGA137800.034.4411967
CGCAGAG141500.033.497968
GTACATG140200.031.2047941
GCAGAGT154250.030.9989769
ACATGGG136850.030.7689783
CATGGGG71750.030.0891084
TACATGG144500.029.8413942
ATGGGGG40950.027.4493795
AGAGTAC147450.025.89396910-11
GAGTACT71650.025.39882312-13
AGTACTT79300.023.81106812-13
CAGAGTA149900.023.70525610-11
TGGGGGG35650.023.0220586
Produced by FastQC (version 0.11.5)