FastQCFastQC Report
Fri 10 Feb 2017
ERR1147818.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147818.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297743
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109440.47629347581518033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64340.2800139093014319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51740.22517748938849994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86050.055.0775871
GGTATCA46850.050.5808561
TCAACGC113750.041.362124
AACGCAG114200.040.938716
ATCAACG115800.040.6812553
CAACGCA115750.040.5446665
TATCAAC123400.038.612942
ACGCAGA132500.035.3294267
CGCAGAG134200.034.878858
GCAGAGT146450.032.0019729
GTACATG130400.029.7702641
TACATGG135300.028.7538172
ACATGGG133600.028.1822033
AGAGTAC138200.026.70383510-11
CATGGGG69600.026.322024
GAGTACT64800.024.91923712-13
CAGAGTA142150.024.85303710-11
ATGGGGG33300.024.8283735
GTATACG1451.334462E-424.637461
AGTACTT68600.023.8423212-13
Produced by FastQC (version 0.11.5)