FastQCFastQC Report
Fri 10 Feb 2017
ERR1147817.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147817.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365702
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126260.5337105011535688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72870.30802696197576873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59410.25113053123343515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91300.052.9483151
GGTATCA49150.050.7524951
AACGCAG115600.041.4217456
TCAACGC115250.041.1346474
CAACGCA117350.040.4492235
ATCAACG117500.040.3469623
TATCAAC125750.037.9837572
ACGCAGA133400.035.804777
CGCAGAG137150.034.825058
GCAGAGT150400.031.9547589
GTACATG133350.031.1176381
TACATGG134650.030.5254482
ACATGGG131400.030.4203533
AGAGTAC143550.026.41434310-11
CATGGGG64650.025.9460324
GAGTACT70450.025.5814112-13
AGTACTT77000.025.2205912-13
CATGGGT25350.024.8724334
CAGAGTA148250.024.63408910-11
CATGGGA57150.022.3775524
Produced by FastQC (version 0.11.5)