FastQCFastQC Report
Fri 10 Feb 2017
ERR1147816.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147816.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405576
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115210.47892895506107475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65950.2741547138814155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54100.22489416256231357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.060.843991
GTATCAA76500.055.789611
TCAACGC103400.040.9622844
AACGCAG104500.040.7588046
CAACGCA105350.040.2605485
ATCAACG105850.040.0703743
TATCAAC116800.036.4673272
ACGCAGA120400.035.4256137
CGCAGAG123300.034.4930468
GTACATG116050.032.519191
GCAGAGT136550.031.3638529
TACATGG119600.031.3359682
ACATGGG118950.030.9564323
CATGGGG64600.028.914914
AGAGTAC127000.027.75007810-11
GAGTACT64250.026.52351412-13
CAGAGTA131600.025.55973210-11
AGTACTT68450.024.24433712-13
GTACTTT73550.023.25063114-15
ATGGGGG33000.021.6317675
Produced by FastQC (version 0.11.5)