FastQCFastQC Report
Fri 10 Feb 2017
ERR1147815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969392
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102000.5179263447805211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61410.31182212581344904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49780.2527683670899445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70850.053.4373971
GGTATCA39600.049.607441
TCAACGC93450.040.424694
AACGCAG95100.039.973546
CAACGCA95650.039.55715
ATCAACG95850.039.2883533
TATCAAC100750.037.671842
ACGCAGA109750.034.5283747
CGCAGAG112900.033.615998
GTACATG109850.031.9727521
ACATGGG110250.031.1890073
TACATGG113750.030.9084592
CATGGGG59300.030.8992774
GCAGAGT125800.030.2161739
ATGGGGG34450.028.8389555
AGAGTAC118500.026.45455210-11
GAGTACT59150.025.04106512-13
GTATACG1200.001284544624.8037191
AGTACTT63350.024.13207612-13
CAGAGTA123200.023.44156810-11
Produced by FastQC (version 0.11.5)