FastQCFastQC Report
Fri 10 Feb 2017
ERR1147814.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147814.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524022
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71350.46816909467186174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43190.28339485912933016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34300.22506236786608066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.065.714991
GTATCAA46950.057.9378741
TCAACGC63750.042.261374
ATCAACG64600.041.70533
CAACGCA65300.041.258235
AACGCAG66850.040.4795386
TATCAAC73500.037.1407742
ACGCAGA77050.034.9664157
CGCAGAG79100.034.132048
GCAGAGT87800.030.8853959
GTACATG76650.027.8781681
TACATGG77600.027.590932
AGAGTAC81850.027.46350310-11
ACATGGG78100.026.348183
CATGGGG44050.025.9227394
GAGTACT37750.025.83507312-13
CAGAGTA85850.024.86777710-11
AGTACTT41000.023.49708412-13
GTACTTT42750.022.81342914-15
CATGGGT20600.022.51924
Produced by FastQC (version 0.11.5)