FastQCFastQC Report
Fri 10 Feb 2017
ERR1147813.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147813.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765780
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78220.4429770413075242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46490.2632830816976067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36000.2038759075309495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56700.057.3423231
GGTATCA28800.056.6531071
TCAACGC71900.044.757574
AACGCAG73750.043.7154926
ATCAACG73450.043.651093
CAACGCA74250.043.2608875
TATCAAC85200.038.051112
ACGCAGA86800.037.1420067
CGCAGAG90100.035.8476648
GCAGAGT98500.032.850999
GTACATG86400.031.152321
TACATGG89100.030.043132
TATAACG1203.7033216E-529.7427022
ACATGGG90550.029.298443
CATGGGG47050.026.9288674
AGAGTAC93700.026.75739310-11
CAGAGTA97100.024.9628510-11
GAGTACT48200.024.24941612-13
ATGGGGG25600.024.1652625
AGTACTT51150.023.66489412-13
Produced by FastQC (version 0.11.5)