FastQCFastQC Report
Fri 10 Feb 2017
ERR1147812.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147812.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617569
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113270.43272975803121144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70420.26902824720188845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55040.21027143888088526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84200.058.2726021
GGTATCA43200.055.4104351
TCAACGC107350.045.3879974
ATCAACG110350.044.1540683
CAACGCA110300.044.1201485
AACGCAG111400.043.951516
TATCAAC124500.039.423972
ACGCAGA127750.038.18677
CGCAGAG132600.036.876898
GCAGAGT145400.033.548689
GTACATG129650.030.3124261
ACATGGG131800.029.4752123
TACATGG132950.029.355622
AGAGTAC135450.027.3606710-11
GAGTACT63800.027.31898912-13
CATGGGG74400.026.787434
CAGAGTA140600.025.87192710-11
ATGGGGG41850.025.0193715
AGTACTT68250.024.31751812-13
CATGGGT29450.024.2412554
Produced by FastQC (version 0.11.5)