FastQCFastQC Report
Fri 10 Feb 2017
ERR1147810.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147810.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736658
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142330.38090186471440524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79570.21294429407240373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64010.17130280587626698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109650.059.2381321
GGTATCA54750.056.111361
TCAACGC142500.044.9169734
AACGCAG145050.044.209356
CAACGCA145300.044.1332865
ATCAACG146150.043.9173133
TATCAAC163650.039.6203042
ACGCAGA167150.038.3636327
CGCAGAG173850.036.95068
GCAGAGT192550.033.4856079
GTACATG177300.031.8335321
TACATGG182300.030.8029732
ACATGGG181200.030.4322913
AGAGTAC179650.028.02633910-11
CATGGGG99850.027.9407394
CAGAGTA186500.025.51395410-11
GAGTACT84150.025.12766512-13
ATGGGGG51000.024.6107835
AGTACTT91000.024.28197712-13
CATGGGA67600.022.175174
Produced by FastQC (version 0.11.5)