FastQCFastQC Report
Fri 10 Feb 2017
ERR1147809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4122682
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126340.3064509947650583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64630.15676688136509195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55720.1351547366495888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113800.057.8192251
GGTATCA57250.048.2608261
TCAACGC145000.044.721414
AACGCAG147750.043.647446
CAACGCA148650.043.4632235
ATCAACG149500.043.375283
TATCAAC167600.039.0459182
ACGCAGA171500.037.5331347
CGCAGAG176650.036.5005048
GCAGAGT199950.032.3958749
GTACATG188450.032.1033131
ACATGGG192400.030.5807573
TACATGG196100.030.4284842
CATGGGG107550.028.4874654
ATGGGGG61800.027.3392665
AGAGTAC184650.027.30002810-11
GAGTACT84250.024.7098912-13
CATGGGT47200.024.4521314
CAGAGTA192650.023.94338210-11
AGTACTT92950.023.6769112-13
Produced by FastQC (version 0.11.5)