FastQCFastQC Report
Fri 10 Feb 2017
ERR1147808.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147808.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520775
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161000.45728568283971566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84650.240430018958894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72130.2048696664796813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116300.061.1244131
GGTATCA56150.058.3180541
TCAACGC153800.045.7508164
ATCAACG156500.044.9615063
AACGCAG157250.044.8983656
CAACGCA157700.044.6570975
TATCAAC168900.041.910092
ACGCAGA186550.037.8778657
CGCAGAG190800.036.9691778
GCAGAGT207250.034.0061349
GTACATG197700.031.620791
TACATGG201750.030.8698442
ACATGGG200950.030.5169493
CATGGGG103200.029.4517864
AGAGTAC199700.028.09901810-11
GAGTACT90100.026.93163112-13
ATGGGGG51700.025.4256555
CAGAGTA203950.025.31181510-11
AGTACTT99550.024.6439312-13
GTACTTT104700.023.23292514-15
Produced by FastQC (version 0.11.5)