FastQCFastQC Report
Fri 10 Feb 2017
ERR1147807.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147807.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883215
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81340.4319209437053125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42530.22583719862044432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35660.18935703039748517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55650.057.6653141
GGTATCA29300.054.6607671
TCAACGC72850.043.523074
CAACGCA74650.042.3939325
AACGCAG75650.042.3053476
ATCAACG75050.042.088723
TATCAAC82100.039.054182
ACGCAGA86850.036.849737
CGCAGAG90000.035.6251458
GTACATG88700.032.1516271
GCAGAGT100700.031.8397529
TACATGG91150.031.1955832
ACATGGG91300.030.7533993
AGAGTAC94550.026.48688710-11
CATGGGG46900.024.6065544
CAGAGTA98450.024.35003910-11
GAGTACT49550.023.28994212-13
CATGGGA38900.022.9384234
AGTACTT54250.021.8204512-13
CATGGGT20650.021.0292954
Produced by FastQC (version 0.11.5)